How long can I keep a Seahorse XFp cartridge hydrated?

More Lorenzo Campanelli's questions See All

How to set up focus groups in the face of generational barriers?

I am carrying out a research project in which I am conducting focus groups with older men and women to study the musical stereotypes learned in traditional Spanish oral music groups, but I am...

10 May 2024 3,852 3 View

Can I use n-hexane as the injection solvent?

mobile phase: methanol/ACN (90:10, v/v) + 9 μM TEA. Can I use n-hexane as the injection solvent? Will this cause column clogging or damage? I know that acetonitrile and n-hexane are immiscible.

05 April 2024 8,861 2 View

Is there a way to extract simultaneously serum and PBMC from a single blood sample?

Hi I'm relatively new to the field, I would like to minimise sample collection from patients but I need to test cytokines and circulating antibodies in serum while also isolating PBMCs If I...

07 February 2024 5,036 3 View

Is there a functional classification in terms of pollination for inflorescences/synflorescences?

I'm trying to analyze a plant-insect interaction data set from a functional point of view, focusing on the architecture of the inflorescences. Do you know of any previous work in which...

06 February 2024 5,803 0 View

Challenges with the optical fiber: Noticing an unusual rise or 'drift' in the light signal when the fiber is bent. What could be the underlying cause?

Dear community members, I need to study the fluorescence of certain analytes in an aqueous solution. Currently, I am investigating fluorescence in a physiological solution. To conduct this study,...

11 November 2023 459 6 View

Can microalgae use EPS/polysaccharides as source of energy?

Hi there. Does anyone know if microalgae (chlorophyta in particular) can use EPS (or polysaccharides) as source of energy? Can you reccomend me any paper where polysaccharides have been used as...

09 November 2023 6,255 2 View

What are the effects of pH on Fluorescence and Absorption of Flavins (Riboflavin, FMN, FAD) and NADH/NADPH in a Simple Aqueous Solution?

Dear all, I am interested in understanding the effects of pH on the spectrophotometric behavior of flavins (riboflavin, FMN, and FAD) and NADH/NADPH (including the oxidized form). Specifically, I...

14 October 2023 4,941 2 View

Partial analysis on agreement studies?

I have a dataset of approximately 170 CT cases. The idea is that the gold standard is the consensus evaluation of two radiologists on 12 descriptive parameters and 1 conclusion. Is it conceivable...

24 September 2023 8,203 1 View

How to Weld/Glue Rhodium on brass support?

Good morning, We are trying to produce ourself a substitute for an X Ray Rhodium anode of a custom setup. We have many cylindrical brass supports, hosting different metal that we use as anodes...

04 September 2023 8,047 0 View

How to calculate the annual radiation dose in ceramics, when there are significant contribution of U, Th, K from both ceramics and sediment?

For dating purposes, to calculate the annual radiation dose in ancient ceramics, the contribution of natural radiation from radionuclides which includes 40K, 238U, and 232Th of the environment...

13 August 2023 9,721 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Assistance with MTT Assay Data ?

I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...

26 July 2024 7,511 3 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

Dartel Ferrari de Lima

72 h

Dimitra Palioura

Hello, did you finally try keeping the cartridge hyrate for more than 72h? Was it ok?

Lorenzo Campanelli

Dimitra Palioura Finally I didnt keep de cartridge for more than 72h.

Thank you Lorenzo Campanelli

Andrea Enriquez

The longest they recommend is 72hrs. I attended one of their trainings recently and they mentioned they don't recommend parafilming cartridges because droplets can leak into the injection ports