How to perform newly synthesized nanoparticles MIC and how to analysis mic dada?
Dear Ashu Devraj, please have a look at the following references. My Regards
https://pubmed.ncbi.nlm.nih.gov/32939454/
https://jnanobiotechnology.biomedcentral.com/articles/10.1186/s12951-015-0120-6
To perform an MIC test on newly synthesized nanoparticles, you will need to:
-Obtain a pure culture of the microorganism you want to test.
-Dilute the nanoparticles in a suitable solvent or media to create a series of concentrations ranging from high to low.
-Prepare a number of tubes or wells in a 96-well plate, each containing a different concentration of the nanoparticles.
-Inoculate each tube or well with a standard amount of the microorganism.
-Incubate the tubes or wells at the appropriate temperature and time to allow the microorganism to grow.
-Observe the tubes or wells for signs of growth or inhibition. The lowest concentration of nanoparticles that completely inhibits the growth of the microorganism is the MIC.
To analyze the MIC data, you can plot the concentrations of the nanoparticles on the x-axis and the growth or inhibition of the microorganism on the y-axis. This will allow you to determine the MIC and visualize the relationship between the concentration of the nanoparticles and their effectiveness against the microorganism.
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