How to remove non-polar impurity from the compound?

I have done the flash column chromatography of the crude mixture. After the slow elution at 4% Ethyl acetate in petroleum ether, the polar impurity is separated and there is not signal of the...

06 July 2017 5,417 3 View

What concentration of the active compound should be used during RT-PCR experiment?

We have performed the anti-proliferation assay and nuclear receptor binding assay and got an IC50 value for the target compounds. I am planning to perform an RT-PCR experiment for analyzing the...

05 June 2017 6,821 2 View

What are the fellowships available for doing PhD in India?

I am doing Ph.D. in Pharmaceutical Sciences and belong to general category. I have 2-year research experience post M.Pharm. Can someone suggest me the fellowship program from Indian Govt. I know...

01 February 2016 8,668 1 View

What can be possible alternative reagent in following mechanism?

In the attached mechanism, what can be an alternative reagent. In this reaction TBBDA (N,N,N,N-tetrabromobenzene-1,3-disulfonamide) has been used.

05 June 2015 5,554 15 View

Why I am getting double the carbon peaks in spectra?

I have separated the compound using flash chromatography and done 2D TLC chromatography for conforming there is no impurity in the separated compound, but still I am getting double the number of...

04 May 2015 1,987 14 View

How to learn QSAR/QPSAR?

i want to learn the QSAR/QPSAR from the medicinal chemistry point of view, I have gone through many books on QSAR all have a tough mathematical background and calculation which i found a bit...

03 April 2015 2,447 5 View

Is there any difference in the abundance of C-13 during season change ?

has any one seen a change in the abundance of C-13 during the winter and summer season, is it possible to observe relatively high abundance of C-13 in a compound during winter as compared to...

01 February 2015 5,973 5 View

Is there any free software/server available for 4D/5DQSAR?

Many a times we need to study the QSAR of the designed molecules/derivatives. Is there is any free software/server available for 4D/5D QSAR study with a good reliability and results are easily...

09 October 2014 7,571 6 View

How does one separate Benzyl bromide from the reaction mixture?

Benzyl bromide and the product formed are soluble in organic phase like ethyl acetate, the excess of benzyl bromide present in the product may lead to its deterioration. How does one separate...

09 October 2014 5,220 36 View

How a medical practitioner can publish his clinical finding in a journal.

A medical practitioner wants to publish his finding and clinical data of a particular medicinal regimen for a treatment of a disorder. what are the key requirement for the publication. As a...

07 August 2014 8,203 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View