Do we need to have both substrate and sealant materials in melt state at the interface for a strong heat seal?
I have been trying to delete one copy of an essential gene from a diploid in fission yeast. I am using PCR based homologous recombination method and primers are designed from Bahler's lab website....
12 May 2024 1,274 4 View
I saw these structures forming in my cell culture. Does anyone know what these are? Have you experienced such a situation before?
04 February 2024 3,791 6 View
We started to see such objects in the flasks containing our canser cell lines? What could these be? Could the cells have been contaminated?
14 September 2023 5,953 0 View
When studying HT-29 colon cancer cells, we observed such a structure in culture. It has a distinct green color. What could it be?
12 September 2023 9,971 0 View
There are different T2A sequences available although all of them share the same protein sequence. Are they codon optimized according to species?
29 July 2022 3,981 0 View
We have DNAse I stock solution (obtained commercially, still lyophilized, should be dissolved in 10 mL according to supplier) and it has been sitting in a closet way too long (nearly 10 years)....
09 June 2022 2,945 0 View
We want to design antisense oligonucleotides for suppressing the gene of interest. The databases or related literatures found are either old or irrelevant. We are new in this field. Are there ASO...
22 March 2022 5,469 0 View
We are planning to transfect HEK293T cells with plasmid that carries shRNA sequence against the gene of interest, grow a large batch and let them produce shRNAs, and then isolate shRNAs. But the...
10 March 2022 1,781 1 View
We want to inhibit gene expression via shRNA in prostate cancer cell lines and then store them for a while for further experiments (apoptosis, proliferation, qRTPCR etc.). I know that it is...
04 January 2022 5,726 2 View
Hi. We are planning to do a single-cell RNA seq in combination with an ONT system for sequencing instead of Illumina. So is there anyone who has done it before? There are a few steps in the...
09 November 2021 5,315 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
How can I measure the thickness of thin film by Uv-Vis?
06 August 2024 7,810 3 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I am seeking experimental or applicable data for the liner (LLDPE) interface in FLAC3D numerical modeling of a large stockpile. Could you please recommend suitable references? The preferred data...
05 August 2024 3,665 0 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View