Are Air-cleaners effective in controlling COVID-19 in indoor environments ?

The use of air cleaners is recommended by some experts to filter the air in indoor environments like home, office, school and other publich places. They have to be equipped with HEPA filters and...

12 November 2020 4,817 3 View

COVID-19 and Geography. What are the levels of impact and who will survive?

Latitude, climate, humidity, infrastructure, health care systems, socioeconomic situation, health condition of the patient and pre-exposure to vaccinations like BCG, antimalarial drugs. Are they...

21 April 2020 6,869 8 View

How to model robot dialogue model according to emotions?

I'm designing a user study on robot-human communication and I want to design the robot speech according to human feelings. I did a research to understand how human feels from speech, and I want...

05 January 2020 7,107 3 View

Is it OK to do a user study with people in a language other than their mother language?

I'm planning to do a user study in Korea, and the study itself is in English. I wanted to hire people with certain English skills, but I was wondering if this is acceptable in academic standards?

05 January 2020 9,293 5 View

What are the main criteria to evaluate an exploration project's success?

How should an exploration project be evaluated as successful or unsuccessful? What are the main criteria? Exploration of an unknown deposit based on recent protocols or standards (such as JORC or...

28 December 2019 460 19 View

What polymers do PNIPAM or Poly(N-vinylcaprolactam) is Miscibile with?

Can you share any relevant atricles? Can you share the curremnt knowledge in this? Any application examples other than gels / membranes? Thank you

11 September 2019 3,926 1 View

How do I continue with my analysis if the Box's M and Levene's tests are significant in MANOVA?

I am trying to run a MANOVA, but the Box's M and Levene's tests are significant. Does this mean I can not run a MANOVA? Is it OK to continue if I choose to report Pillai's trace in stead of Wilks'...

01 January 2017 4,851 11 View

How to make Tris Succinate buffer (0.05M, pH8.2)?

How to make Tris Succinate buffer (0.05M, pH8.2)?

25 October 2016 5,036 3 View

Any suggestions about my struggle with fabrication of silver coated core shell nanoparticle?

29 August 2015 5,565 8 View

Is Matrigel or GFR-Matrigel better for transwell invasion assay?

I will be working with NCI-H460 cells.

02 February 2015 9,969 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View