I have miR-222-3p, miR-125b-5p in case of OAS3 as our gene of interest, and TRAFD1 as Transcription Factor all in case of humans as host. All these are forming a 3-node Feed-forward loop (i.e., gene, TF, and miRNA) and I want to understand the molecular biology behind this. We just know that OAS3 is upregulated in our study.
Please clarify if which of my hypothesis is/are correct:
1) Is the TRAFD1 (TF in our case) is repressing or activating both the miRNAs which in turns upregulates the OAS3 as an antiviral gene to the site of Hepatitis E virus (HEV) infection? If this is the case then what should be the expression state of both miRNAs and TFs.
2) Do both miRNAs (being downregulated) are actually upregulating OAS3 with TF also upregulating or activating OAS3? If this is the case, then how all these are functioning.
3) Does both TF and miRNAs jointly work together to upregulate OAS3? If this is the case, then how all these are functioning.
Please explain the correct scenario along with TF and miRNAs possible expression status. We only know OAS3 is upregulated.
Thanks....
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