• I have been preparing competent dh5alpha cells in the lab with good competency not excellent. however, have not been able to transform my CRISPR plasmid yet. I am following all the desired steps still unable to attain the correct colonies. plz, throw some light where I can be making mistakes. Plasmid is from addgene (pSpCas9(BB)-2A-Puro (PX459) V2.0)
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