How do you determine the novelty of STR markers?

More Negar Fakhari's questions See All

What's the better material instead of Glutamine for CHO cells in cell culture?

I've designed media for CHO cells, without Glutamine! But when they're in large scale, the cells were damaged and decreased their viability. What's your refer ?

14 January 2024 2,503 2 View

How can I find PCR markers and suitable antibody to flow cytometry of C2C12 cell line?

How can I find "PCR markers" and "suitable antibody to Flow cytometry" of C2C12 cell line? Is there any web server to find this data?

02 December 2023 3,248 0 View

Can someone provide me with the earthquake repositioning code with Python?

29 September 2023 6,469 3 View

Would using a high ROX master mix cause any issue for PCR instruments that do not require ROX as normalizer?

Hi ResearchGate community, I have been trying to learn more about the optical differences between block-based real-time PCR machines like ABI StepOne versus rotor-based machines such as MIC or...

22 July 2023 964 6 View

How long can transient plasmids (like PEGFP N1) remain after transfection into bone marrow stem cells?

hello, I have a major question that i would really appreciate if anyone could make me clear. In a project I am going to transfect BMSCs with a plasmid by lipofectamine 2000 reagent. then the...

06 June 2023 9,768 1 View

Can I import bipolar electrode montage into eeglab?

I want to use an epilepsy database ( CHB-MIT Scalp EEG Database) and the samples have some bipolar channels like FP1-F7, F7-T7, T7-P7, P7-O1, FP1-F3, etc. We can define monopolar channels in...

12 July 2022 3,546 0 View

Can we extract intracellular cellulose from bacteria which exist in paper mill activated sludge?How?

I dont mean bacterial cellulose(the cellulose which produce by bacteria like G.xylinus ) or the cellulose fibril that present in paper mill activated sludge, i mean a protocol for extracting the...

02 July 2022 6,182 2 View

Is there any fully automatic MR prostate segmentation?

Is there any free available tool or even demo that gets the MRI image and creates the contouring of different organs? I need to just test some few cases. Thanks.

07 March 2022 1,689 0 View

How to mature "Oligodendrocyte precursor cells (OPCs)" CELL-LINE to "Myelin producing Oligodendrocytes"?

Hello everyone I am working on a human cell-line of Oligodendrocyte Precursor cell (OPCs) called MHB. Results of my immunocytochemistry experiments showed that these cells express NG2, and don’t...

15 December 2021 3,208 1 View

With the NCBI Probe Database being retired, is there any similar database I can refer to for finding tetra nucloetide microsatellite markers?

I have been searching the publications reporting STR markers in the genomic neighborhood of my interest, but I wonder if there might be a more straightforward approach to it. Also within the UCSC...

23 November 2021 8,236 4 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Katie A Burnette

I'm assuming you're working in humans? Try the Online Mendelian Inheritance in Man (OMIM) database and NCBI.

Negar Fakhari

Hi Katie A Burnette,

Many thanks for your response.

Yes, I am working on a cohort of CF patients.

Can I ask for further directions on where exactly to navigate to the related section in those databases?

Best,

Muhammad Umair Dear Muhammad,

Thank you so much for your kind reply.