How do we check the concentration of oligonucleotide after resuspending it in NFW??

20 February 2023 0 2K Report

I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm but its not coming to desired concentration.. tried different formulas also but not getting through.. is there any way to check this?? please reply

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Science method

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