How can I set up for 2 photon imaging to look at Dendritic spine morphology in live 500 micron section from GFP mouse brain?

Akshata A Korgaonkar @Akshata-Korgaonkar-2

15 December 2017 0 1K Report

Interested in looking into dendritic spine morphology in deeper areas of (250 micron deeper from surface) live brain sections which are constantly perfused with ACSF. Typical imaging time for one slice would last for couple of hours. Need help with slice health( Cutting solution, bath solution detail for thicker section), Perfusion setup, 2 photon imaging parameters ( objectives, media, scanning resolution etc.)

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