How to check RNAse contamination in consumables and materials in house??
How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.
07 January 2015 7,046 9 View
We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.
02 January 2014 123 2 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
What should one do if he obtains conflicting results between Content Validity Index (CVI) and CVR for items in a quetionnare. For example based on the CVI obtained the items are acceptable but the...
25 February 2021 7,325 2 View
I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...
23 February 2021 7,868 2 View
Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...
22 February 2021 6,126 2 View
Knowing that the Venturi effect is used most in tubes (according to my basic knowledge in physics) to increase the speed of air or liquids by reducing the tube volume, my question is if we have...
17 February 2021 1,260 1 View
Hi, I am experiencing some difficulties with 3T3-L1 cells culturing recently. I am working with 3T3-L1 cells for almost 4 years now and have seen bacterial contaminations/ unhealthy cells and...
13 February 2021 4,040 3 View
I am working on a biosensing project on paper substrate, however, it is found that the RNase contamination occurs on the filter paper. Might I ask is there any method to clean the paper surface...
12 February 2021 3,926 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...
07 February 2021 8,258 3 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View