Akshata Helwande

6 Questions 2 Answers 0 Followers

Questions related from Akshata Helwande

Phenol red preparation?

i want to use phenol red for LAMP assay but its not getting dissolved in NFW. Which other solvent can be used to dissolve the same and will not affect the LAMP reaction?

04 August 2023 3,955 3 View

How do we check the concentration of oligonucleotide after resuspending it in NFW??

I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm...

20 February 2023 8,900 2 View

How do we check the concentration of oligonucleotide after resuspending it in NFW??

I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm...

20 February 2023 1,554 0 View

How do we check the concentration of oligonucleotide after resuspending it in NFW??

i want to quantify my oligos as a part of incoming inspection. We used spectro for quantification and tried different formulas to check the concentration but none of them are nearby 100uM. Kindly...

01 January 1970 1,680 2 View

Concentration of quencher and probe

At what nanometer should we take the OD of quencher and probe?

01 January 1970 4,624 0 View

Rnase Contamination check

How to check RNAse contamination in consumables and materials in house??

01 January 1970 7,503 0 View