Akshata Helwande

3 Questions 1 Answers 0 Followers

Questions related from Akshata Helwande

How do we check the concentration of oligonucleotide after resuspending it in NFW??

I have resuspended my primers in NFW to get the final concentration of 100uM. Now i want to confirm that the concentration is 100uM.. how can i do that?? i have tried spectrophotography to confirm...

20 February 2023 8,838 2 View

How do we check the concentration of oligonucleotide after resuspending it in NFW??

i want to quantify my oligos as a part of incoming inspection. We used spectro for quantification and tried different formulas to check the concentration but none of them are nearby 100uM. Kindly...

01 January 1970 1,677 2 View

Concentration of quencher and probe

At what nanometer should we take the OD of quencher and probe?

01 January 1970 4,621 0 View