The unidentified substance of my interest derived from a plant is only methanol soluble. how would I do MTT assay using this substance of my interest?
Dissolve it in methanol but do keep in mind that the final concentration of methanol should be less than or equal to 1% you can also use DMSO to dissolve your substance and here also the the concentration of DMSO should not exceed 1%
The substance gets dissolved from the lipid extract of the plant only when 90% methanol is used
So take that as your vehicle, but do remember it should not exceed 1% of the entire contents. You dissolve your substance like 1mg in 10ul of 90% methanol and then add 990ul of incomplete media of your respective cell line to make the drug with 1mg/ml concentration.
Good Luck!
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