Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

Micro CT of fiber reinforced composite - phase identification?

Hi, I have a fiber reinforced polymer matrix composite and I am analyzing it by micro CT but I am unable to reconstruct the fiber phase of the composite. how can I find a solution for this?...

07 January 2021 5,680 1 View

Dear all, Is there any model available to predict the "possible diseases" based on the available pollutant concentration in air ?

In detailed human health risk assessment models. Is there any model that can predict the possible disease.

21 December 2020 7,535 5 View

Is calcination necessary for synthesis of Pd deposited CeO2 nanomaterials?

I want to synthesize Pd deposited CeO2 mixed metal oxide composites

03 December 2020 3,195 6 View

Would there be any difference in transfection efficiency of plasmid eluted from mini and maxi prep?

24 September 2020 8,198 3 View

Dispersion of Graphene?

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14 September 2020 8,364 5 View

How to reduce STL file size for simulation study?

I have created a lattice structure using Autodesk Netfabb and saved it as a .stl file. I want to export it as .iges to ANSYS to conduct FEA but the file size is too big and it crashes. When I try...

27 March 2020 4,136 3 View

How to input a particular value of frequency using ABAQUS FEM software?

Dear fellow scientists, I am working on a project which is in correlation with acoustic imaging using ABAQUS. I am having trouble inputting a particular value of...

05 August 2019 8,865 8 View

Band broadening in sequencing gels: How to avoid?

Hello all, I have been running sequencing gels for quite some time now and I find that the bands start broadening as the dye reaches the halfway mark (pic attached). I followed the publications...

03 November 2018 4,008 8 View

Do Protein A/G agarose beads nonspecifically bind to DNA?

Hi all, I am performing a Co-IP experiment where would like to check the binding of my protein to the DNA of interest. I kept a control where I incubated the DNA and antibody only with the...

08 May 2018 6,193 5 View

No title

23 February 2021 4,014 3 View

Why is there violent fluctuation of ThT signal in Amyloid beta aggregation?

Dear all, I am currently working on amyloid beta aggregation. In ThT monitered aggregation assay, I have already obeserved an increase in ThT signal, indicating the aggregation of amyloid beta....

15 February 2021 4,852 2 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Can I heat my ELISA at 37C?

Hello, I am conducting a sandwich-type ELISA. It is to see if small molecules can interfere with a protein-protein interaction. As this interaction would occur in the human body, may I heat...

31 January 2021 4,271 3 View

Why can I not see any plaques when performing a viral plaque assay with MHV?

28 December 2020 5,515 2 View

Coronal or Sagittal mouse brain sections to visualise vessels?

Hello, I am a first year PhD student and I am working on Cerebral amyloid angiopathy (CAA). I would like to investigate the presence of my proteins of interest in a CAA/Alzheimer's disease mouse...

05 December 2020 907 3 View

Can anyone recommend me your cloning protocol that successfully works in your laboratory?

Dear fellow scientists!!! I have been doing cloning lately and have encountered some problems during my experiments, for example, I can get none or very few colonies after transformation into...

02 December 2020 2,271 11 View

Did we interpret our SDS-PAGE results correct?

This is our first time doing SDS-PAGE, and therefore we would like to hear from more experienced people if we interpret the results right. Attached is a picture of one of our gels with our...

01 December 2020 1,440 3 View

Are 96-well plate adhesive seals necessary in broth microdilution MIC assays?

I am going to perform a minimum inhibition concentration (MIC) assay using the broth microdilution method which will involve a 12-16 hour incubation period of the 96-well plate in a shaking...

19 November 2020 5,928 3 View