Cellulose is broken down by the enzyme cellulase, to find the cellulase activity and unit production by the microorganisms, the microorganisms were cultivated by using cellulose substrate CMC and OD value were calculated and also whole protein production by the microorganism also calculated. then How to do cellulase enzyme production by microorganisms calculation having a glucose standard y = 0.0013x - 0.0906, total protein of that organism = 77.24 µg/mg, and cellulase OD 540nm value of that particular organism taken at 0th min = 0.5106, 1st min = 0.5126, 2nd min =0.5159 and 3rd min = 0.5166, I want to find the unit of enzyme catalyzes 1 µmol of substrate per min, and activity in (U/ml) and also specific activity in (µmol/min/mg)? [where epsilon coefficient for glucose = 3.2x10(-4)]?
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH