i am planning to do ELISA for AB quantification in an Alzheimer related project. the sample is cell culture (not animal samples), since AB deposits in the extracellular environment should i use only the supernatant or the cells?
Hi Gita Parviz ,
It depends on the type of your cells. Hippocampal cells (CA1) for example can internalize beta amyloid. I would recommend to homogenize your sample and use both cells and supernatant.
Best
Hello Gita Parviz
You may use both cell lysate as well as conditioned media (supernatant).
Best.
If it is secreted and extracellular concentration is sufficient to perform the experiment, adding lysate-originated proteins means a more complicated sample and increased vulnerability to interference. I just prefer the secretome to be analyzed if AB is completely secreted into media. You may verify it first by quantifying them separately (intracellular and extracellular).