Albert Pilkington

6 Questions 21 Answers 0 Followers

Questions related from Albert Pilkington

How does NH4OH or NaOH disaggregation protocol for Abeta actually break up existing beta sheet structures?

I understand the process behind why fluoro alcohols break up existing secondary structures, but for the alkaline conditions (NH4OH or NaOH) used in some protocols how are the basic conditions...

17 August 2015 4,610 8 View

Amyloid Road Block and really need HELP!!!?

I need some help with my beta-amyloid protocol/ ThT assay. I’m getting it to work occasionally but the results are not reproducible to the point they need to be. When the assay works well the...

06 July 2015 1,142 10 View

Can anyone share Thioflavin T storage and oxidation conditions?

Okay this one is a pretty easy question, but I'm curious how other labs are storing their ThT salt. I get varying information from companies. We have been storing ours at RT in cabinet (bottle is...

19 August 2014 8,407 3 View

Why am I getting <1000 fluorescence intensity in my thioflavin T assay?

I know that the intenisty of ThT can vary from assay to assay when monitoring amyloid fibrills, but my intensities are well below what I feel they should be. The intensities range from 0-200,...

04 July 2014 9,173 24 View

Can anyone help with my method for Tris buffer and Abeta fibrils?

Has anyone successfully seen Abeta fibrils in a Tris buffer within 72hrs? I'm currently doing ThT assays with 20uM Abeta40, Tris buffer, 20uM ThT and @ 37C for 72hrs. I'm not seeing anything and I...

05 June 2014 9,196 5 View

Methods to quantify extent of lipid oxidation in total brain lipid extract (TBLE)?

I'm looking to titrate total brain lipid extracts with hydrogen peroxide. Then under fluid AFM introduce amyloid-beta to determine changes in aggregation profiles with respect to the extent of...

26 May 2014 6,273 3 View