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Questions related from Albert Pilkington
I understand the process behind why fluoro alcohols break up existing secondary structures, but for the alkaline conditions (NH4OH or NaOH) used in some protocols how are the basic conditions...
17 August 2015 4,611 8 View
I need some help with my beta-amyloid protocol/ ThT assay. I’m getting it to work occasionally but the results are not reproducible to the point they need to be. When the assay works well the...
06 July 2015 1,143 10 View
Okay this one is a pretty easy question, but I'm curious how other labs are storing their ThT salt. I get varying information from companies. We have been storing ours at RT in cabinet (bottle is...
19 August 2014 8,408 3 View
I know that the intenisty of ThT can vary from assay to assay when monitoring amyloid fibrills, but my intensities are well below what I feel they should be. The intensities range from 0-200,...
04 July 2014 9,174 24 View
Has anyone successfully seen Abeta fibrils in a Tris buffer within 72hrs? I'm currently doing ThT assays with 20uM Abeta40, Tris buffer, 20uM ThT and @ 37C for 72hrs. I'm not seeing anything and I...
05 June 2014 9,197 5 View
I'm looking to titrate total brain lipid extracts with hydrogen peroxide. Then under fluid AFM introduce amyloid-beta to determine changes in aggregation profiles with respect to the extent of...
26 May 2014 6,274 3 View