How do I convert the weight of plasmid DNA in kilo base pairs to daltons?

More Aishwarya Saraswat's questions See All

Is aliquoted basic fibroblast growth stable at 22 degree Celsius for how long?

Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable

15 June 2024 3,010 2 View

What could this structure be?

We were imaging platelets under SEM. We got this structure. Any recommendation on what this structure could be will be of great help. Thank you

02 May 2024 3,320 4 View

Require Biochemical (Quantitative) assay to detect Albumin and casein protein?

Looking for biochemical assays for quantitative estimation of Albumin and Casein protein.

28 April 2024 4,285 3 View

High mRNA expression versus low protein expression?

I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with up-regulation at mRNA level and down-regulation at the protein level....

05 April 2024 4,679 6 View

Why the paddy seeds not germinating?

My paddy seeds are not germinating properly. I have tried different methods to break dormancy like- hydropriming, Dry heat (45 degrees for 2 days), and acid treatment (1.5% HNO3) at 30-32 degree...

01 January 2024 7,000 6 View

How do I solve this?

I have been getting these long smeared lanes for very long in western blot. Every time I make fresh buffers and even I have changed the antibody brand. Nothing helped. Please provide suggestions...

05 November 2023 6,888 4 View

Inquiry regarding the thermal kinetics observed in a Differential Scanning Calorimetry (DSC) analysis?

I have a query is that after crystallization peak point some of other noise type peaks occur in DSC scan for (Se78-xTe20Sn2Znx) x=2 and x=6 ,this peak shown the material property or due to...

05 August 2023 8,945 1 View

I used Spe1 and BamH1 restriction enzymes. Why is the 1st band smeared?

I used Spe1 and BamH1 restriction enzymes for checking presence of plasmid in EP1 300 strain. It had a 40kb insert size. I have got faint bands at 6kbp and at 2kbp. But the first band is smeared....

03 August 2023 7,901 0 View

After gel extraction i see faint additional bands of just double in size of expected band i.e 900 bp.Why?

Pcr product on agarose is clear with one sharp expected band size of 900bp but at the after dna purification i observed double bands of exactly double size on agarose

26 July 2023 7,831 0 View

Can we use debaryomyces hansenii as an expression vector?

I want to use yeast as an expression system. So, I wanted to ask if Debaryomyces hansenii could be used for the same. Although, Saccharomyces is a model system for gene expression studies. Can we...

26 July 2023 3,742 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

Darya Meshalkina

Hi, Aishwarya. To get approximate meaning you'll need to remember, that every base pair weights approximately 660 Da. So, if you multiply 5.7 thousands base pairs on 660 Da, you'll get the approximate answer, that is applicable in most cases.

Shiva Kumar

Kindly check wid d attached file

Katie A Burnette

MW of a double-stranded DNA molecule = (# of base pairs) X (650 daltons/base pair)

This will give you the weight of a SINGLE DNA molecule of a specific size. it does NOT account for the number of DNA molecules in a solution.

Mallikarjuna Reddy Kesama

https://www.promega.com/~/media/files/resources/technical%20references/plasmid%20and%20protein%20quantitation.pdf

https://www.genscript.com/conversion.html

http://www.molbiol.ru/eng/scripts/01_06.html