Can we use debaryomyces hansenii as an expression vector?

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Is there any good online workshop on advanced DEA models?

OR ANY study material with step by step analysis & its interpretation using the software?

01 January 2021 993 1 View

How do I get ConsistencyDupSNP.sh file required for genotype consistency quality control step of genotyping data obtained from GSA 24 v2.0 ?

Genotyping data is from Illumina Global screening array 24 v 2.0 and ConsistencyDupSNP.sh file contains list of pair of duplicated SNPs in GSA24 v 2.0.

18 December 2020 1,966 3 View

Can I get some important resources to analyse Photoluminescence Spectra?

I am trying to figure out what information can I get with the help of PL spectra and how to analyse it? How to do the defect analysis, if I am using two different material for thin film how will...

14 November 2020 2,453 6 View

Hazard value for malaria survival graph??

In an experiment untreated infected mice and infected treated mice observed. I recorded my data in terms of survived mice to 28 days in each group. How to calculate hazard value for treatment vs...

06 November 2020 5,548 1 View

How to analyze nominal/ordinal data in cases where you are testing multiple drugs/ infection against common risk factors?

chi-square or Fisher exact test I have a statistical question about chi-square and/or Fisher exact test. I have a cohort data studying the cause of infection in BRD with variables such as age,...

28 October 2020 2,589 1 View

What tips to follow while renaming a research paper in your repo for easier and quicker retrieval later during Ph.D research?

We often download tons of research paper related to our Ph.D research domain in their default filename format. After few days, recollecting and finding a particular paper becomes difficult. Any...

12 October 2020 2,425 3 View

Why will a very intense peak in XRD even beyond instrument detection limits, will appear in a Sample?

I recently submitted my samples in two different labs, I am getting three very intense peak in between 69-70 (2theta degrees); I want to know what could be the possible reason behind, is it normal...

13 September 2020 2,064 4 View

What kind of data ICPOES Perkin Elmer Optima 5300V ICP-OES provide with?

I recently submitted my samples to a laboratory for ICPOES, I am only provided with Standard Deviation and Mean data of one element out of two. I want to know if there is any spectra that is...

21 August 2020 2,428 2 View

How to check virulence of Fusarium oxysporum TR4?

Please add your suggestions for the following questions: 1. Can anyone suggest me the meaning of "fungus has lost its virulence"? 2. If I am able to observe micro and macroconidia under...

27 July 2020 1,099 1 View

Why do drug resistant cell lines show different morphology?

I have a HNSCC cell line resistant to a drug (2 different clones) showing different morphology than the parental cell line. Attached are phase contrast images of these cells. There are black dot...

15 July 2020 1,881 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View