Pcr product on agarose is clear with one sharp expected band size of 900bp but at the after dna purification i observed double bands of exactly double size on agarose
can anyone suggest me the book to know the answer to the above question? the graph has been attached
07 September 2020 7,512 4 View
the Root least square method with forgetting factor for implementation in MATLAB i have an ARX model for parameter estimation. How to calculate the P(0) for this method ?
15 June 2020 2,168 2 View
Nitrobenzene is sparingly soluble in water. I tried plotting its standard curve with various dilutions ranging from 0.1mM - 1mM, using HPLC with the method given in papers (Mobile phase: Methanol...
17 March 2020 5,306 3 View
While working on tools like STAR CCM+, why AHMED body is considered to be the basic of 3D CAD modelling & what are it's applications in the real life industry??
03 February 2020 3,521 3 View
Please provide some free SCI/SCIE/SCOPUS indexed journal for fuzzy group.
30 December 2019 5,952 1 View
I have been trying to rescue a gene knock out phenotype by complementing with the same gene under a plasmid, but the rescue I am getting is close to 30% . I have tried differential promoter...
24 March 2019 2,432 2 View
I want the properties of exhaust gases like viscosity, thermal conductivity, pradantal number at 4 bar pressure. Heat Mass data book mostly gives these values at 1 bar pressure.
06 February 2019 1,440 6 View
I am studying effect of stress on protein-protein interaction. For that control cells are stored at -80C without any stress. However, Before performing protein purification, Can cells be stored...
12 December 2018 4,152 1 View
As MMAS-8 is copyrighted, and the authors does not give permision to use it , is there any validated Medication Adherence Scale/ tool which is free to use in research or academic purposes?
11 September 2018 174 3 View
I have send my research article in many Scopus index journals but most of them rejected by citing reason that article missing innovation and add value to research knowledge base.
21 August 2018 9,251 9 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View