Pcr product on agarose is clear with one sharp expected band size of 900bp but at the after dna purification i observed double bands of exactly double size on agarose
how to calculate dosage for cell culture treatment by using plant extract ?
12 May 2024 7,335 1 View
Looking for most cost-effective manner to outsource my samples (silver stained gels) for proteomics analysis using LC-MS/MS. If anyone has outsourced samples outside or in INDIA for LC-MS/MS and...
21 April 2024 309 2 View
I had transformed my desired gene along with selectable marker into rice plants and started with gene expression studies. Previously Amplification was observed in both genes but now on repetition,...
14 March 2024 7,841 1 View
Hi, Is there any free application I can use to study root morphology? The following information would be helpful to me: primary and lateral root lengths, root density, and root area. Thank you
11 January 2024 6,401 2 View
my protein is a nucleolar protein which is tagged with HA, FLAG and myc tag. i have over-expressed it and good GFP expression was observed which is directly proportional to the amount of my...
11 January 2024 2,057 2 View
My paddy seeds are not germinating properly. I have tried different methods to break dormancy like- hydropriming, Dry heat (45 degrees for 2 days), and acid treatment (1.5% HNO3) at 30-32 degree...
01 January 2024 7,000 6 View
Despite having negligible photorespiration and photosynthetic advantages, what are the factors or reasons that C4 minor millets failed to surpass the yield of C3 rice ?
10 December 2023 702 1 View
How to obtain the permission from original author for translation of scale?
27 September 2023 7,521 2 View
I want to know about the orange node and brown mid rib traits observation in forage pearl millet
06 September 2023 3,220 0 View
I have data on number of tillers,number of tillers at both cuts and tillers regrown i need any supporting paper and supporting answer whether there exact formula like that
06 September 2023 2,756 0 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View