How could one deliver a chemical fluorophore inside a yeast for imaging?

20 December 2018 1 5K Report

I am looking forward to label proteins inside yeast cell. I wonder if there are chemical fluorophores one could use in yeast for imaging?

If yes what are the methods by which it could be delivered inside the cells?

Adam B Shapiro

The usual way is to make fluorescent protein chimeras. Here is another way, but I don't know if it works with yeast:

https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cellular-imaging/high-content-screening/flash-and-reash.html

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