Athul M Sivan

7 Questions 1 Answers 0 Followers

Questions related from Athul M Sivan

Is there a cell permeable dye against His-tag that one could use for live cell labeling and imaging?

I am trying to tag proteins with several tags and will be interested in exploring the dyes that can be used for live-cell imaging. His-tag in particular is an interesting candidate for my...

05 July 2021 2,189 2 View

How could one deliver a chemical fluorophore inside a yeast for imaging?

I am looking forward to label proteins inside yeast cell. I wonder if there are chemical fluorophores one could use in yeast for imaging? If yes what are the methods by which it could be delivered...

20 December 2018 5,199 1 View

What are some good markers for C2C12 myotubes?

I am trying to differentiate C2C12 myoblast cells. I would like to study the myotubes and hence requires some markers for myotubes

16 January 2017 1,246 2 View

Where does microRNA actually bind?

Does it bind to just 3' UTR region of mRNA or both 3’ and 5’ ends of mRNA ? Will it bind to other coding regions?If it binds to multiple sites, which of them is responsible for gene regulation?

14 July 2014 2,061 7 View

What are actually novel miRNAs?

As a beginner in molecular genetics I have come across and got confused with the term novel-miRNA many times. But I can't figure out what it really is.

10 June 2014 7,830 1 View

Can anyone explain how to work with RNAhybrid in cygwin?

I am here to find the miRNA target sites. I could do that online via RNAhybrid web service. But I have no idea how to work with RNAhybrid in cygwin.

05 June 2014 5,262 1 View

Drawbacks of Lifeact in live cell imaging

LifeAct is a 17aa peptide sequence that could be conjugated with a fluorophore that could bind actin. Though Lifeact is being widely used for live cell imaging, it is reported that there are...

01 January 1970 2,713 2 View