I have to test mycoplasmas in cell cultures, and they asked me to prepare a medium cmm Culture. I do not have the protocol to prepare this medium cmm.
Hello , I'm asking you why they consider that Wilcoxon test and U Mann Whitney test are the same ? Can we use this test to examine 2 numeric variables for independent groups ? Thank you
08 September 2018 8,735 3 View
Hello, I am conducting a research on the key domains of a different protein sequences. Can you give me the link of the best software that I can use to do this task ?
11 December 2017 5,865 3 View
hello, i want to know why according to the literature when they establish standard curves they use cloned bacterial DNA (for exemple for feacalibacterium strains) in a plasmid and for other...
04 May 2017 1,245 2 View
Hello, I want to sequence rRNA16S of bacteria by means of next generation sequencing technic using Illumina MiSeq technology. In articles i see that they choose hypervariable region V4 or V6...
11 December 2016 1,564 1 View
Hello, I want to compare data related to nutrition of people to standard ones in order to see if diets are hyper or hypo caloric...Can you help me in order to find standard norms of nutrition...
11 December 2016 1,311 0 View
Hello, I have 500 SNPS (single nucleotide polymorphisms) of cases and controls individuals, if i will use snpassoc (tool r studio) it will take too much time to insert variants, what is the best...
07 August 2016 6,241 1 View
Hello, I want to know when can we use Student's test, Pearson's correlation coefficient test and Spearman rank correlation, what can i put us matrix (numeric variables as means..sd (standard...
04 May 2016 4,542 7 View
Hello, I conserved the extracted microbial DNA from kit in -20°C (in TE BUFFER), after that i was obliged to transfer the microbial DNA to + 4°C because i can't ensure the transport from a place...
03 April 2016 6,935 3 View
Hello, For the SNP rs5219, i looked for the GMAF, i found T=0.2630/1317 but HGVS names indicated NC_000011.10:g.17388025T>C, What can i do in this case, there are some contradictions.? Thanks
02 March 2016 8,678 1 View
Hello, I need the definitions and utilities of each biostatistics tests quoted and the tutorial (tool r/matrix models) in order to achieve that. I need also the definitions and utilities of each...
10 November 2015 8,540 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View