On which criterion is based the choice of the hypervariable region of bacterial rRNA16S to do NGS?

12 December 2016 1 2K Report

Hello,

I want to sequence rRNA16S of bacteria by means of next generation sequencing technic using Illumina MiSeq technology. In articles i see that they choose hypervariable region V4 or V6 ..knowing that they are 9 hypervariables regions in rRNA16S.I want to know on which criterion is based the choice of the hypervariable region of bacterial rRNA 16S ?

Thank you

Yu-fei Lin

This Nature review may provide some insight as to which variable region to choose.

http://www.nature.com/nrmicro/journal/v12/n9/pdf/nrmicro3330.pdf

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