I want to detect and measure approximately amount of lysine with TLC, how can I measure it (lysine), can anybody introduce method or software?
Please open this data base, you will find many methods for determination of lysine by using TLC:
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm?method=query
Hi dear Krysztof, thank u for useful answer, how can I use OD for measuring amount of lysine? Which OD? can you introduce one article about that?
Regards
If you have HPTLC system you can prepare concentrations from standard lysine and apply the sample spot on the same plate, after development an scanning the software will produce calibration curve and measure the amount of your sample directly.
While doing TLC we have to run standard with the experimental sample and run them using appropriate solvent. The spot will be developed on TLC plate . Then when it gets dried , take it out from the TLC plate and mix in a solvent and the use Gas Liquid Cinematography (GLC) or U V ray or HPLC to get the peak which is compared with standard and then you may identify it and measure the quantity.
Long back during m Ph.D work , I did it for Alkaloid.
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