16 Questions 39 Answers 0 Followers
Questions related from Fereshteh Sadat Younesi
I am doing polysome fractionation for stress conditions for mammalian cell. it seems that peaks of 40S and 60S disappear during stress condition compared to control. I am wondering whether all...
13 November 2018 3,720 2 View
With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...
20 August 2017 1,206 6 View
I have two enzyme, one of which is full enzyme and the other is truncated ( small domain containing 85 aa is deleted from full enzyme containing 537 aa). I need concentration of purified enzymes...
04 August 2015 7,728 10 View
my enzyme is thermophile and has two site binding Ca. I want to examine the role of these Ca in stability and activity. what is a range of concentration of EDTA used?
23 July 2015 9,700 6 View
I want to detect and measure approximately amount of lysine with TLC, how can I measure it (lysine), can anybody introduce method or software?
19 April 2015 673 4 View
When enzymes were eluted with high concentration immidazole, some of them lost their activity in 2 days. I want to know how immidazole affect enzyme or other protein. I can't find article about that.
20 January 2015 7,413 6 View
I know definition of Km and Kcat. when affinity of enzyme to substrate in the two enzyme ( wild_type and without small domain) haven't had any change, how does the rate of catalysis increase? My...
17 January 2015 2,091 4 View
my enzyme is cellulase. It has two domains( small domain and catalytic domain). small domain has a few hydrophobic interaction with catalytic domain. I determined PH optimum for truncated and...
20 December 2014 8,087 3 View
I removed small domains from my enzyme. I want to know which one (truncated or wild-type) is more likely to aggregate at different times at 65 centigrade. Can you give me a strategy to go about...
01 December 2014 9,123 9 View
For understanding of the function of domain, I removed small domain ( with 7 beta sheet) from my enzyme. my enzyme( cellulase) has two domain that the catalic domain is major domain. I check...
14 October 2014 5,774 2 View
I provide mix buffer containing 100 mM glycine, 100 mM tris, 100mM succinic acid and 100 mM imidazole with ph 3, 3.5.....9 25 microlitr enzyme in tris buffer with ph 7.5 was mixed with 50...
14 September 2014 251 19 View
I use Tris-HCL as lysis buffer for thermophilic enzyme. Is it possible the PH of this buffer changes when I use high temperature for enzyme assay?
07 August 2014 1,907 14 View
My gene inserted in pET21(+) vector. T7 promoter can be induced by lactose. What is the best condition for reaching high production with lactose?
07 August 2014 2,322 3 View
I don't understand what the relation is between amount of OD and time of induction.For example my recombinant protein is expressed well when I induce bacteria in OD=1. While another protein was...
10 July 2014 9,308 12 View
I purified my protein with 250 mM imidazole, after 2 days, I remove imidazole with dialysis, but I can see a white pellet in protein. My protein is an enzyme, it has activity immediately after...
26 May 2014 6,544 17 View
I purified my enzyme with 250 mM imidazole by ni-agarose column, my enzyme has 2 metal in structure ( Ca and Zn). Is it necessary to add this metal into buffer dialysate?
24 May 2014 7,127 1 View
