I am interested to know how can I modeling Troposphere with Bernese software? It means that can you introduce a source for it?

I readed the Bernese manual which that published at Bernese website, but it was'nt enough.

10 March 2020 6,098 3 View

Have you ever seen that 40S and 60S of ribosome subunit peaks disappear from polysome fractionation?

I am doing polysome fractionation for stress conditions for mammalian cell. it seems that peaks of 40S and 60S disappear during stress condition compared to control. I am wondering whether all...

12 November 2018 3,513 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

Has anybody transfected an integrative plasmid DNA into rat bone marrow-derived mesenchymal stem cells by lipofectamine 2000 with a high efficiency?

I am working with rat BM-MSCs, and i am trying to transfect these cell with integrative pIRES-EGFP plasmid by applying lipofectamine 2000 (invitrogen). however I found these cells very hard to...

31 August 2018 9,851 3 View

How residential satisfaction and sustainability could be related to each other?

i need some references which have discussed how residential satisfaction may lead to different aspects of sustainability.

20 June 2018 7,713 3 View

Which subunit is the best one to measure for assaying NF-kB activation via Real time-PCR?

I want to investigate NF-kB activation pathway using Real time-PCR. is Real time-PCR a valid method for this purpose and if it is, what is the best subunit to measure?

14 October 2017 3,525 2 View

Does anyone know how to scoring Three factor eating-18 questionnaire?

I have some question about questionnaire scoring. I would be grateful if anyone kindly guides me in these questions. My questions are:If The response Likert for questions 14 to 17 are different...

25 September 2017 5,040 3 View

What is the best solvent to dissolve Perylene-d12?

30 August 2017 1,493 1 View

What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

19 August 2017 1,026 6 View

Cooper nanoparticle

28 December 2016 7,410 6 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

How can you tell if your organotypic slice culture is healthy ?

I'm working on organotypic cerebellar slice cultures from postnatal rats and I'm not sure how to tell if the slices are healthy at any given point. I know there should be a lot of microglial...

15 February 2021 2,830 2 View

Is it possible to perform cardiac puncture followed by PBS perfusion with a pump?

Hello all, I will be perfusing mice with PBS for brain harvest and a colleague would like to collect the blood via cardiac puncture (these are 18m old mice so would be nice to maximize use). Has...

15 February 2021 5,312 2 View

Why daily dexamethasone given to rats fed high fat/fructose/salt cause very high mortality rate?

rats were given daily dexamethasone in drinking water corresponding to 0.5 mg/kg or 2 mg/kg. These rats are simultaneously fed 25% fat, 3% salt in diet and 10% fructose in drinking water but...

15 February 2021 7,634 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

How do I fix tissue rolling while cutting frozen rat brain section with a cryostat?

I am cutting rat brain sections at 20um at -20C. The tissue is cutting nicely out of the O.C.T solution, but I can not get a decent section to adhere to the slide. I have included an image.

09 February 2021 1,345 5 View