Can anyone tell me how passing spheroids? We collected them,through 40um mesh filter, and recovery the spheroids from the filter adding PBS. After that, the spheroids are centrifuged and disaggregated with trypsin or accutase. But we thought that we lose spheroids in the filter.
We want to analyze them by flow cytometry.
Thank yoyu.
Guillaume Vidal
Hi,
why don't you pellet them with (slow if necessary) centrifugation ?
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