What areas of cardiac imaging do you think are prone to automation processes?

Cardiac imaging procedures are growing exponentially and their complexity are increasing even further. We do not have time, money and personal resources to face with this enormous demand. Machine...

10 November 2017 9,006 3 View

How long is sectioned brain tissue good for something?

Bird brains cryosectioned and kept in cryoprotectant in a -20C freezer for ~5 years. It was preserved with acrolein and some sections were used for IHC right away. What can I do with the rest of...

23 October 2016 9,173 6 View

What is the most effective trap for sampling wood-boring beetles (Buprestidae)?

I am interested in collecting adult wood-boring beetles (Buprestidae) in deciduous cropping systems. The purple prism or panel traps have been effective for monitoring emerald ash borer (Agrilus...

08 August 2016 1,066 5 View

What does it mean to have a wide peak at 400 nm and a narrow peak at 300 nm for silver nanoparticles?

Hello everybody, I would like to make a question about the UV-VIS spectrum of silver nanoparticles. I started a project to synthesize silver nanoparticles in the plant extracts and I have two...

13 July 2015 992 7 View

I'm looking for pathological heart rate data for testing a cloud simulator. Anyone aware of data about this?

05 October 2014 4,688 2 View

Could someone help me with details to measure the particle size of CdSe fluorescent quantum dots using a DelsaNano C particle analyzer?

I am using a DelsaNano C to quantify the size and distribution of CdSe quantum dots (narrow size distribution ranging from 1 to 20 nm). Until now I have not succeeded. I am sure I have quantum dot...

25 March 2013 4,177 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View