Guillaume Vidal

4 Questions 44 Answers 0 Followers

Questions related from Guillaume Vidal

Quickly pellet floating fatty cells in culture plate?

Hi, I have fatty floating cells in culture medium and I need to simply/quickly pellet them without degrading cells nor removing themfrom the plate. Any idea ? thanks

03 March 2018 1,338 4 View

ROS and viability quantitation on the same sample.

I'd like to image ROS accumulation in cells with a fluo dye and I'm wondering if anyone have ever performed cell lysis to mesure ATP after ROS imaging ? Does the ATP quantitation is still reliable ?

06 June 2017 674 2 View

Mitochondria 2D vs 3D ?

Could anyone provide me some fundamental informations/data about mitochondria in 2D vs 3D mamallian cell culture (activity, regulation, stress responses, ROS production...)?

10 October 2015 2,576 1 View

Which nuclear dye should I use, as I can't use DAPI or Hoechst?

I'm doing LSCM observations on fixed cells. I can not excite DAPI nor Hoechst dyes. Does anyone can suggest me a nuclear dye that is not emitting near the Alexa488 and 594, to prevent overlapping...

08 August 2015 1,838 4 View