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06 May 2019 3,491 3 View

Method for statistical analysis of semiquantitative Western-Blot?

Hello, I need some advice about how to statistically analyze WB data. I am trying to compare the levels of two proteins in mutants relative to wt samples (it's mouse embryonic tissue). I...

10 February 2019 6,052 3 View

What areas of cardiac imaging do you think are prone to automation processes?

Cardiac imaging procedures are growing exponentially and their complexity are increasing even further. We do not have time, money and personal resources to face with this enormous demand. Machine...

10 November 2017 9,006 3 View

How long is sectioned brain tissue good for something?

Bird brains cryosectioned and kept in cryoprotectant in a -20C freezer for ~5 years. It was preserved with acrolein and some sections were used for IHC right away. What can I do with the rest of...

23 October 2016 9,173 6 View

What is the most effective trap for sampling wood-boring beetles (Buprestidae)?

I am interested in collecting adult wood-boring beetles (Buprestidae) in deciduous cropping systems. The purple prism or panel traps have been effective for monitoring emerald ash borer (Agrilus...

08 August 2016 1,066 5 View

How can we harvest spheroids without filtration?

Can anyone tell me how passing spheroids? We collected them,through 40um mesh filter, and recovery the spheroids from the filter adding PBS. After that, the spheroids are centrifuged and...

10 November 2015 8,777 1 View

What does it mean to have a wide peak at 400 nm and a narrow peak at 300 nm for silver nanoparticles?

Hello everybody, I would like to make a question about the UV-VIS spectrum of silver nanoparticles. I started a project to synthesize silver nanoparticles in the plant extracts and I have two...

13 July 2015 992 7 View

I'm looking for pathological heart rate data for testing a cloud simulator. Anyone aware of data about this?

05 October 2014 4,688 2 View

Could someone help me with details to measure the particle size of CdSe fluorescent quantum dots using a DelsaNano C particle analyzer?

I am using a DelsaNano C to quantify the size and distribution of CdSe quantum dots (narrow size distribution ranging from 1 to 20 nm). Until now I have not succeeded. I am sure I have quantum dot...

25 March 2013 4,177 1 View

When is the best incubation time of the bacteria, to know the dry mass ?

Mostly the incubation time of bacteria is 48 hours. But, if i want to know the dry mass of the bacteria. So, how long i should incubated it? is it 24 hours, 48 hours, or in the mid log phase?...

01 January 1970 3,648 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Can anyone help me with the administration of the "Mind the Gap" Scale for satisfaction with transition to adult care?

We are working on a study focused on adolescents' transition to adult care and are looking for a validated measure for satisfaction with the transition. So far the only measure that we have came...

28 February 2021 2,338 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

Is there an open source free software for text mining and sentiment analysis?

I am looking for a free software for text mining and sentiment analysis for my research on customer review mining (it involves calculating polarity of attributes,opinion oriented information...

25 February 2021 1,920 6 View

Which are the journals for free paper publication?

A bunch of flower for everybody. I would like to ask about the list of journals which we can publish our paper for free. Thanks in advance. Sincerely Walid

25 February 2021 9,790 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View