It may be not exactly right. But I think if you can use Beer-Lambert law, there can be a possibility as it considers the relationship between concentration and absorbance.

Dear Mohan

You can drow a linear relation ship between optecl density of the solution and graident concentration of the materials up to reach the maxiumum value hich should be below 2. but the lowest is the better. then by excel program you can drw a linear relation ship between the optical density (Absorpance) and concentration. The regression equation of the trind line can be used to calculate the molar concentration at any absorbence within thr range.

I wish you Alla Success.

Yasser El-Nahhal

IUG

Using Beer's law to get straight line (calibration curve) which is the dynamic range, then you can get the value of concentration by knowing the absorbance.

Hutaf M. Baker

I agree with Hutaf M Baker

I think this link is useful, 

http://www.instanano.com/characterization/theoretical/concentration-calculation-from-uv-vis-absorbance/

I have the same question, if you find the answer could you guide me