How can I produce cell line from human primary fibroblast cells, extracted from synovial Tissue?

I am working on human fibroblast cells from synovial tissue and now they are in 4-5 passages. These cells have been confirmed with specific markers (99% purity). Recently, I have decided to...

17 November 2020 627 3 View

How can I get the diameter (molecular size) of the drug?

Diclofenac is separated by a nanofiltration membrane and the molecular size of diclofenac is needed to prove the mechanism of size exclusion. I did not find the molecular size of diclofenac...

29 October 2020 7,604 2 View

How do you eliminate and control internal bacterial contamination in plant tissue culture?

Hi dear fellows I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination. I tried ppm and antibiotics in media but both of them have...

20 September 2020 8,143 4 View

Is there a monotectic reaction in Fe-Cu system?

Hi all, In Munitz's paper attached, it is explained on Page 9 that there is a monotectic reaction in Fe-Cu alloys (Fe based alloys), but I cannot see where on the phase diagram the monotectic...

15 September 2020 5,358 4 View

How to define an objective function with an index (j) in GAMS?

Hello everyone, is there any method to define an objective function with an index (j)? I cannot define the objective functions as the number of the indexes due to the objective functions are...

13 January 2020 8,560 7 View

What is a purification method to remove urea from peptid solution (no dialysis)?

I want to remove remove urea from peptide solution (elution solution from gel filtration column).The solution is 6M urea and 20mM tris (pH 7). I am trying to avoid dialysis as there are many...

31 December 2019 168 4 View

Is there a free software to find the peaks of the FT-IR chart?

I need a software to find the peaks and draw the FT-IR chart of my research. I have essential FT-IR but it is not free.

18 April 2019 7,879 4 View

Anyone can help me with locating a water/wastewater treatment using Nanotechnology in their treatment process?

There are lots of laboratory studies on nano application in water/wastewater treatment. however not sure if there is any real world treatment plant sample using this kind of technology in its...

08 April 2019 3,554 7 View

How does temperature influence reflectivity and absorption?

25 July 2017 2,250 8 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How the wettability of rock sample can be changed by ageing in pure hydrocarbon oils only?

I want to do wettability analysis of hydrocarbon oil (octane)/rock/surfactant solution system. but to do the analysis first I have to make rock surface oil-wet. Since hydrocarbon oil does not have...

27 February 2021 5,383 3 View

How the values of errors can be calculated for thermodynamic data?

I have calculated thermodynamic parameters for surfactant solutions in aqueous and mixed alcoholic solvents. I want to calculate the value of errors in the data and represent it in the table along...

22 February 2021 239 2 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Does any one have experience with Autoclavable cell culture media (RPMI 1640/DMEM)? Does it work well?

12 February 2021 2,958 1 View

How to determinate of molecular structure of salt into the liquid?

I need to determine the open formula of our complex, presumably consisting of sodium tungstate Na2WO4 and aluminum sulfate Al2 (SO4) 3. Our problem is that with an increase in the concentration...

11 February 2021 5,821 1 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Why does sodium metabisulfite generate turbidity when mixing with sodium hydroxide?

When I tried to mix a sodium metabisulfite solution with sodium hydroxide solution, the mixture solution quickly became turbid. Before mixing both were clear solutions. Please help explain the...

03 February 2021 8,075 2 View

I want to extract engine oil from a soil sample,please how much amount of anhydrous sodium sulphate am i going to add to 10kg soil?

Engine oil extraction from contaminated soil,10kg of contaminated soil+20ml of dicholoromethane+? of anhydrous sulphate

01 February 2021 9,509 1 View

What is the best procedure for measuring the pH of aqueous colloidal systems?

There has long been a debate among my colleagues about measuring the pH of aqueous colloids. As I see there is no general truth in the matter, so I ask this question below. What is the best and...

31 January 2021 8,203 3 View