Why does gel in phase lock tubes come as the upper layer?

26 April 2023 0 9K Report

I used phase lock tubes for RNA extraction, but after adding chloroform and spinning down the gel came on top of aqueous layer! does anyone know why this might happen? and what should I do to inhibit this?

More Mehrnaz Zarinfar's questions See All

I want to make a lysing solution to lysis RBC and WBC in the cell counter device but with the human blood the WBC is all in lymph. what should I do?

I need to make a lysing solution to lyse the RBC and WBC for using in cell counter of sysmex k1000. When I use the blood control all parameter are normal but when I used the human blood the cell...

11 February 2024 8,536 0 View

How to quantify Sodium Lauryl Sulfate content in the distilled water?

I have a solution containig some salts and also Sodium Lauryl Sulfate (SLS). How can I identify the quantity of this surfactant?

13 July 2023 9,195 2 View

How to encounter endogenous bacterial contamination?

Dear fellows I am into micropropagation of calladium. we didn't observe any bacterial contamination during the initiation and first subculture stages. the second subculture was done last week and...

09 August 2022 6,391 3 View

Why i can not replicate PDMS mold out from master with nanoholes?

I have a master with nanohole with diametre ~ 200 nm and a depth of 150 nm. i can not replicate the PDMS mold out from this master, every time PDMS is attached and fill the holes... Does anyone...

08 May 2022 1,143 0 View

What is a solution for "recovery" of certain types of elements from seawater by using a membrane? As an example, the recovery of just Lanthanum?

-The atomic radius of lanthanum is approximately 0.35 nm, as opposed to 0.3 nm for water molecules. -Seawater sample also contain organic contaminants such as Mg, Na, etc. some of them with the...

02 January 2022 6,673 4 View

2% Methyl Cellulose being separated after autoclave?

Hello everyone! I'm trying to make 2% MC, but after I autoclave the solution the water and the gel phase separate from each other! What can be done about this? Can I put it again on stirrer to...

21 July 2021 7,568 3 View

How can I get the diameter (molecular size) of the drug?

Diclofenac is separated by a nanofiltration membrane and the molecular size of diclofenac is needed to prove the mechanism of size exclusion. I did not find the molecular size of diclofenac...

29 October 2020 7,792 2 View

How do you eliminate and control internal bacterial contamination in plant tissue culture?

Hi dear fellows I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination. I tried ppm and antibiotics in media but both of them have...

20 September 2020 8,423 4 View

How do you do calculation for preparing silver thiosulfate?

hi every body. silver thiosulfate (STS) is an ethylene inhibitors that enhance shoot organogenesis. the following is the preparation of STS according to sigma aldrich web site: Prepare a 0.1...

17 July 2020 4,486 1 View

Suspended air flotation in industrial wastewater treatment?

Hi, I am doing technical due diligence for a technology so-called Suspended air flotation in industrial wastewater treatment. this technology is similar to DAF, but using froth instead of air...

15 April 2020 2,392 0 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View