Mohan Amarasiri

10 Questions 21 Answers 0 Followers

Questions related from Mohan Amarasiri

How can I export the draft genome sequence created by MAUVE as a FASTA file for downstream applications?

I want to construct the whole genome sequence of a bacterium isolated in our lab using the order/move contigs option of MAUVE. I used the reference genome and the program provided me two...

28 June 2016 4,343 1 View

How do I confirm false positives in flow cytometry?

I want to isolate some antigen positive bacteria from an environmental sample and planning to use FACS for the process. As positive and negative controls I have used bacteria which confirmed to be...

22 October 2015 2,656 22 View

Why do dead bacteria have higher fluorescence in flow cytometry?

I am using molecular probes red bacterial stain in flow cytometry. Can anybody explain how the dead bacterial cells can have higher fluorescence intensity?

10 July 2015 2,275 4 View

How do I the stain bacteria and antigens in the same sample?

I want to detect the presence of bacteria and antigens from the same sample using two fluorescent stains with flow cytometry. How can I double stain the sample for that purpose?

22 April 2015 8,291 6 View

How can I roughly convert the available median and percentile data into mean and SD values?

I have extracted some median value and percentile data from publications and I would like to convert them to mean and confidence interval data for a comparison. What are the simple statistical...

20 January 2015 8,879 6 View

What is the best temperature to store feces samples before bacteria isolation experiment?

I want to store some feces samples for bacteria isolation. I am planning to store them in -800C. Will it be ok? How long can I store that way without destroying the bacteria?

08 December 2014 1,655 4 View

Can I use western blotting to measure polysaccharides if I use polysaccharide standards?

I want to measure polysaccharide concentrations and the size. Can I use western blotting for that purpose?

07 December 2014 8,423 2 View

What is the effect of periodate treatment on bacterial cells?

I am treating bacterial cells with periodate for degrading the glycoproteins. Will it effect the viablility of cells?

29 November 2014 2,478 2 View

How long can I store influent, effluent, and mixed liquor in the freezer without degredation of bacteria and viruses?

I want to do virus concentration measurements and I am planning to store my samples in the -80 C freezer. How long can I keep them without degradation of the bacteria and viruses?

15 January 2014 6,869 0 View

What is the LPS concentration inside a MBR?

A typical Lipopolysaccharide concentration inside a MBR is required for an experimental design. Are there any typical values which can be assigned for the initial design?

09 January 2014 6,960 1 View