I have a master with nanohole with diametre ~ 200 nm and a depth of 150 nm.
i can not replicate the PDMS mold out from this master, every time PDMS is attached and fill the holes... Does anyone have the same problem? or did have experience the same?
I need to make a lysing solution to lyse the RBC and WBC for using in cell counter of sysmex k1000. When I use the blood control all parameter are normal but when I used the human blood the cell...
11 February 2024 8,536 0 View
I have a solution containig some salts and also Sodium Lauryl Sulfate (SLS). How can I identify the quantity of this surfactant?
13 July 2023 9,195 2 View
I used phase lock tubes for RNA extraction, but after adding chloroform and spinning down the gel came on top of aqueous layer! does anyone know why this might happen? and what should I do to...
25 April 2023 8,718 0 View
Dear fellows I am into micropropagation of calladium. we didn't observe any bacterial contamination during the initiation and first subculture stages. the second subculture was done last week and...
09 August 2022 6,391 3 View
-The atomic radius of lanthanum is approximately 0.35 nm, as opposed to 0.3 nm for water molecules. -Seawater sample also contain organic contaminants such as Mg, Na, etc. some of them with the...
02 January 2022 6,673 4 View
Hello everyone! I'm trying to make 2% MC, but after I autoclave the solution the water and the gel phase separate from each other! What can be done about this? Can I put it again on stirrer to...
21 July 2021 7,568 3 View
Diclofenac is separated by a nanofiltration membrane and the molecular size of diclofenac is needed to prove the mechanism of size exclusion. I did not find the molecular size of diclofenac...
29 October 2020 7,792 2 View
Hi dear fellows I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination. I tried ppm and antibiotics in media but both of them have...
20 September 2020 8,423 4 View
hi every body. silver thiosulfate (STS) is an ethylene inhibitors that enhance shoot organogenesis. the following is the preparation of STS according to sigma aldrich web site: Prepare a 0.1...
17 July 2020 4,486 1 View
Hi, I am doing technical due diligence for a technology so-called Suspended air flotation in industrial wastewater treatment. this technology is similar to DAF, but using froth instead of air...
15 April 2020 2,392 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
These fungal slides are isolated from cultured Bread mold on PDA media and smear is treated with Lacto phenol Blue dye ..
13 July 2024 9,701 1 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,375 2 View
I use PDMS to build a microfluidic system, but it is not hardening at all, although I use TEOS as a cross-linker and also ATPES in a ratio of 1:10, and it's been 3 days at 80 degrees of 3 ml cast...
01 July 2024 604 3 View