Hi dear fellows
I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination.
I tried ppm and antibiotics in media but both of them have negative effect on multiplication and growth of explants. As I am a mass producer of these two plants this contamination costs me a lot every year. do you have an idea that can help in controlling and eliminating endogenous bacterial contaminatin?
Dear Mehrnaz Falaki it's a topic with great concern to TC, since the endophytics occuring is ubiquitous to in vitro culture. Its solution is possible but not easy. Many factors must be considered as well as your scopes. What kind of antibiotics have you used? Regarding PPM, I have used it without success; however, many other researches used it frequently to obtain clean cultures.
Clean cultivation with use of resistance varity may be best option to minimize internal bacteria. proper fertilizer dose also important for bacteria.
Antibiotics like streptomycin have been used against bacterial contamination in plant tissue culture e.g. Nystatin can be used as sterile solution to rinse contaminated ex plants .
If you explain about the type of explant, the concentration, type and method of antibiotic were used, and the ppm concentration, better and more accurate guidance can be given.
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