2% Methyl Cellulose being separated after autoclave?

More Mehrnaz Zarinfar's questions See All

I want to make a lysing solution to lysis RBC and WBC in the cell counter device but with the human blood the WBC is all in lymph. what should I do?

I need to make a lysing solution to lyse the RBC and WBC for using in cell counter of sysmex k1000. When I use the blood control all parameter are normal but when I used the human blood the cell...

11 February 2024 8,536 0 View

How to quantify Sodium Lauryl Sulfate content in the distilled water?

I have a solution containig some salts and also Sodium Lauryl Sulfate (SLS). How can I identify the quantity of this surfactant?

13 July 2023 9,195 2 View

Why does gel in phase lock tubes come as the upper layer?

I used phase lock tubes for RNA extraction, but after adding chloroform and spinning down the gel came on top of aqueous layer! does anyone know why this might happen? and what should I do to...

25 April 2023 8,718 0 View

How to encounter endogenous bacterial contamination?

Dear fellows I am into micropropagation of calladium. we didn't observe any bacterial contamination during the initiation and first subculture stages. the second subculture was done last week and...

09 August 2022 6,391 3 View

Why i can not replicate PDMS mold out from master with nanoholes?

I have a master with nanohole with diametre ~ 200 nm and a depth of 150 nm. i can not replicate the PDMS mold out from this master, every time PDMS is attached and fill the holes... Does anyone...

08 May 2022 1,143 0 View

What is a solution for "recovery" of certain types of elements from seawater by using a membrane? As an example, the recovery of just Lanthanum?

-The atomic radius of lanthanum is approximately 0.35 nm, as opposed to 0.3 nm for water molecules. -Seawater sample also contain organic contaminants such as Mg, Na, etc. some of them with the...

02 January 2022 6,673 4 View

How can I get the diameter (molecular size) of the drug?

Diclofenac is separated by a nanofiltration membrane and the molecular size of diclofenac is needed to prove the mechanism of size exclusion. I did not find the molecular size of diclofenac...

29 October 2020 7,792 2 View

How do you eliminate and control internal bacterial contamination in plant tissue culture?

Hi dear fellows I am working on micropropagation of Begonia and Gloxinia which in both of them have internal bacterial contamination. I tried ppm and antibiotics in media but both of them have...

20 September 2020 8,423 4 View

How do you do calculation for preparing silver thiosulfate?

hi every body. silver thiosulfate (STS) is an ethylene inhibitors that enhance shoot organogenesis. the following is the preparation of STS according to sigma aldrich web site: Prepare a 0.1...

17 July 2020 4,486 1 View

Suspended air flotation in industrial wastewater treatment?

Hi, I am doing technical due diligence for a technology so-called Suspended air flotation in industrial wastewater treatment. this technology is similar to DAF, but using froth instead of air...

15 April 2020 2,392 0 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

What should be the sample container for the hydrothermal reaction in a microwave reactor at 180 °C for 10 min at the heating rate of 5 °C per minute?

Suggest one of them 1. Teflon-lined stainless steel autoclave: 2. Alumina (Al2O3) ceramic container

30 July 2024 7,326 1 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

I want to buy Hydrothermal Synthesis Autoclave from any European company. Can anyone suggest any company inside Europe?

Thanks

22 July 2024 1,143 3 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View