How to increase the 260/280 and 260/230 ratios in DNA extraction?

06 May 2024 1 6K Report

I manually extract DNA using a modified Sambrook protocol (phenol-chloroform), from tissue samples (skin or muscle) of stranded animals even at a high state of decomposition. When I take Nanodrop readings to check the extraction yield, although the quantity is high, the ratios are low, especially the 260/230 one. How can I obtain cleaner DNA?

Péter Gyarmati

Use Qubit or Bioanalyzer (or quantitative gel electrophoresis if nothing else) for your measurements if you can, phenol residues can give false positives with Nanodrop.

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