Homogenization for Chlorophyll Analyse?

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Do you have a tried protocol for GSH measurement?

Do you have a tried protocol for GSH measurement? I will use it for wheat.

17 September 2023 2,984 0 View

Is product of ideal topology is ideal topology or not?

Let (X, tau, I) be an ideal topology I ask: wThe product of this topology itself is ideal topology or not?

21 August 2023 6,122 4 View

How can ı measure chlorophyll content?

I'm going to measure chlorophyll spectrophotometrically. Does anyone have a tried-and-tested, detailed protocol?

14 July 2023 4,910 4 View

How can I write Ridge Quantile Regression codes in R package program?

Can I write codes for ridge quantile regression in R package program? Are there any resources for this? What are your recommendations? Thank you for your answers in advance.

29 May 2023 7,046 3 View

Which buffer is available for GSH calculation?

I use the Ellmann method for the determination of glutathione. There are so many options as a buffer. I prepared a 0.1 molar 8.4 pH Tris buffer for the reaction mixture. I have a trismza base...

23 May 2023 2,501 2 View

How to solve this error in SPH to SPH LS-Dyna impact analysis ?

I am trying to simulate an hypervelocity impact analysis between spherical projectile and composite fabric including fiber and matrix. (All of them are SPH) Elastic plastic hydro material and...

12 May 2023 3,971 1 View

How can i tackle this problem to obtain the damage on my LS-Dyna simulation ?

I am trying to simulate an hypervelocity impact between a SPH sphere and FEM composite material which is a combination of fiber and matrix. After the impact instead of damage or erosion i obtain...

12 May 2023 8,020 0 View

GSH absorbance values are negative???

I did a GSH measurement on the samples today in -80. I used the Ellman method. Reaction comparison: 0.1 ml sample, 2 ml Tris 8.4, 0.1 ml Ellmann reaction. i measured it at 412 nanometers, but my...

13 April 2023 7,633 2 View

How can ı measure GST activity?

I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.

23 January 2023 5,204 3 View

Calculation of protein???

I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?

17 January 2023 2,199 2 View

Request a single Lecture notes for math as detailed as this that I can find in one place?

- The Existence/Uniqueness of Solutions to Higher Order Linear Differential Equations - Higher Order Homogenous Differential Equations - Wronskian Determinants of $n$ Functions - Wronskian...

03 August 2024 2,366 0 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View

Does soybean seed coat or cotyledon contain chlorophyl or flavonoids? What types? determined by paper chromatography? other methods (high school lab)?

Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.

25 July 2024 7,498 2 View

Why my plant protein cannot resolubilize after TCA/Acetone extraction?

I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...

15 July 2024 9,025 3 View

How does the melting point of a spherulite correlate with its size?

For Polybutylene Terephthalate (PBT) it is known that if you cool it down at fast rates to have an additional melting Peak in DSC-Scans. Androsch et al. (2015) conclude that this is due to...

30 June 2024 2,891 1 View

His column crack?

Hi... I seem to be in a very big trouble. My His columns look cracked after my last purification. In another column, the materials seem to be pushed. How is it possible and is there anything I...

24 June 2024 7,071 2 View

Inquiry Regarding Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT)?

Dear All, I am writing to solicit your expertise regarding the application of Higher-Order Shear Deformation Theory (HSDT) and First-Order Shear Deformation Theory (FSDT) for structural...

22 June 2024 7,751 5 View

Effect of post bake on PPR resist?

HI , i am concerned about any resist hardening effect of Post bake my LOR +PPR coated sample @90C for 5 mins. I wanted to do liftoff in acetone but the metals are hard to peel off. I cant use PG...

18 June 2024 5,741 0 View

What analysis can be performed to detect traces of acetone in extracts?

Use a solution of acetone and water to perform an extraction. After performing it, evaporate the acetone and lyophilize the extracts, but I am looking for a methodology to confirm that acetone has...

10 June 2024 5,047 3 View

Replacement for acetone in dehydratization of cryo sections?

We are currently trying to replace acetate when dehyrdating ("drying") cryo sections on glass slides with a less harmful alternative. For many chemical purposes ethyle lactate is recommended - has...

10 June 2024 5,213 3 View