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Do you have a tried protocol for GSH measurement? I will use it for wheat.
18 September 2023 2,945 0 View
I use the acetone method for chlorophyll analysis. Should I do homogenization after making an addition with acetone?
10 September 2023 6,491 2 View
I'm going to measure chlorophyll spectrophotometrically. Does anyone have a tried-and-tested, detailed protocol?
15 July 2023 4,868 4 View
I use the Ellmann method for the determination of glutathione. There are so many options as a buffer. I prepared a 0.1 molar 8.4 pH Tris buffer for the reaction mixture. I have a trismza base...
24 May 2023 2,472 2 View
I did a GSH measurement on the samples today in -80. I used the Ellman method. Reaction comparison: 0.1 ml sample, 2 ml Tris 8.4, 0.1 ml Ellmann reaction. i measured it at 412 nanometers, but my...
14 April 2023 7,602 2 View
I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
24 January 2023 5,144 3 View
I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?
18 January 2023 2,178 2 View
I want to measure protein content in my plants. So, ı will use Lowry method. How can ı prepare extraction buffer for plant materials?
19 December 2022 8,427 0 View
I'm studying heavy metal toxicity in wheat. I need to prepare a standard for GSH measurement, and I could not determine the appropriate GSH standards. Does anyone have any information?
16 October 2022 4,845 3 View
