I did a GSH measurement on the samples today in -80. I used the Ellman method. Reaction comparison: 0.1 ml sample, 2 ml Tris 8.4, 0.1 ml Ellmann reaction. i measured it at 412 nanometers, but my absorbance values are negative. Where am I going wrong?
Niti Sharma
It seems your samples are diluted. Try using concentrated ones. Are you getting a proper standard curve?
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Rohit S Ranabhat
I used Gpx assay from cayman chemicals, and the curve goes down. Could be calculations?? I don't use the Ellman method. You could be measuring the change in reaction rate(absorbance).
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