Do you have a tried protocol for GSH measurement? I will use it for wheat.
I use the acetone method for chlorophyll analysis. Should I do homogenization after making an addition with acetone?
09 September 2023 6,533 2 View
Let (X, tau, I) be an ideal topology I ask: wThe product of this topology itself is ideal topology or not?
21 August 2023 6,122 4 View
I'm going to measure chlorophyll spectrophotometrically. Does anyone have a tried-and-tested, detailed protocol?
14 July 2023 4,909 4 View
Can I write codes for ridge quantile regression in R package program? Are there any resources for this? What are your recommendations? Thank you for your answers in advance.
29 May 2023 7,045 3 View
I use the Ellmann method for the determination of glutathione. There are so many options as a buffer. I prepared a 0.1 molar 8.4 pH Tris buffer for the reaction mixture. I have a trismza base...
23 May 2023 2,500 2 View
I am trying to simulate an hypervelocity impact analysis between spherical projectile and composite fabric including fiber and matrix. (All of them are SPH) Elastic plastic hydro material and...
12 May 2023 3,970 1 View
I am trying to simulate an hypervelocity impact between a SPH sphere and FEM composite material which is a combination of fiber and matrix. After the impact instead of damage or erosion i obtain...
12 May 2023 8,019 0 View
I did a GSH measurement on the samples today in -80. I used the Ellman method. Reaction comparison: 0.1 ml sample, 2 ml Tris 8.4, 0.1 ml Ellmann reaction. i measured it at 412 nanometers, but my...
13 April 2023 7,633 2 View
I'm going to measure the GST enzyme activity spectrophotometrically. Does anyone know the procedure? The absorbances I measured turn out to be at a negative value. I used the Habig method.
23 January 2023 5,204 3 View
I used Lowry Technic. I prepared standards from BSA for make up standard curve. And then, ı measured my samples proteins. How can ı find protein contents in my samples?
17 January 2023 2,198 2 View
Hi there, My question is: What standard curves should be used while estimating Tot GSH and GSSG by kinetic method using GR enzyme mediated recyling with DTNB chromophore? Actually I am following...
01 August 2024 8,217 1 View
I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?
28 July 2024 4,884 2 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,886 0 View
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
20 June 2024 3,225 2 View
How top dressing of nitrogenous fertilizers can be done in mulched wheat and rice crops and how should FYM/compost be applied in zero till wheat?
02 June 2024 3,836 3 View
We need to produce hybrid wheat seed for our experiment. Ca anyone tell me what's the best chemical agent for this purpose and where to buy the chemical agent? Thank you for your help.
27 May 2024 833 2 View
I am a researcher of Soil science department. 5 months ago I had planted wheat for my research. The research design was split plot design. it has 3 replications, each replication has 2 main plot...
05 May 2024 4,815 3 View
Hi everyone, I'm trying to extract fungal DNA from wheat common bunt using chelex100 and I've tried several different protocols without any success. I've used different concentrations of chelex100...
29 April 2024 7,317 1 View
28 April 2024 7,431 5 View
Hello, My lab recently acquired a combination ORP electrode, with the refrence being Ag/AgCl and the working electrode being platinum. We are hoping to use this electrode to measure the...
26 April 2024 6,914 1 View