How can I specifically biotinylate Proteins in either N terminal of C terminal when my protein is stable at pH 7.5can any body suggest me protocol ?

03 June 2025 1 8K Report

Stability of protein is at Ph 7.5 ?

How can we control and only have site specific biotinylation ?

Dear Kandluri Geethika

you can add at the C-term or N-term an Avi-TAG sequence (GLNDIFEAQKIEWHE) that could be specifically biotinilated using a kit containg BirA enzime.

https://www.genecopoeia.com/product/biotin-protein-ligase/

it is quite simple and it work well for mg scale of protein.

best

Manuele

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