I am developing a protocol for the generation of antibodies using the Phage display method. Could anyone share a protocol for bio panning of biotinylated antigens?
have a look if you find the desired information here:
J. Stolz et al., FEBS Letters 440 (1998) 213-217
We used biotinylated antigen for M13 phage display in the following paper with good outcome:
https://www.nature.com/articles/s41598-020-79036-0
Thank you Thomas.
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