Changing Aspen Plus non-conventional component into a mixture of few conventional compounds?

I have performed a bio-refinery simulation on Aspen Plus using non-conventional components. Now, what I am willing to do is to specify one of the product (non-conventional) into a mixture of some...

12 February 2020 5,469 4 View

How analysis of variance for augmented design can be done conveniently??

22 April 2016 6,287 3 View

Why is the user subroutine terminating the analysis?

i am getting an error ***ERROR: Analysis is being terminated from a user subroutine. Apparently i cannot pinpoint the exact source of the error. I am not sure but i can just guess that its due to...

25 February 2016 2,220 1 View

Can we find refractive index by using Kubelka-Munk Function [F(R)]?

can we find refractive index by using Kubelka-Munk Function [F(R)]??

26 December 2015 4,737 2 View

How can I analyse NGS data of microbiome (For beginner)?

I am beginner in the field of Bioinformatics and started analysing NGS data of microbiome. I would like to know what is the best software available to start analzsing sequencing files from MiSeq....

09 June 2015 4,351 10 View

What is the ideal gene for internal control in real-time PCR? Is is 18SRNA or G6PD?

Hi, What is the ideal gene for internal control in real-time PCR? 18SRNA or G6PD? I am working on the Leukemia(AML) patient samples(bone marrow/blood..RNA extraction), and when I do PCR on cDNA...

19 January 2015 2,198 7 View

Is it Zagrammosoma or Cirrospilus sp., need help with the ID?

1.8 mm parasitic wasp from the larva of apple-leaf miner Lyonesia clerkella

01 January 1970 7,577 3 View

How can I calculate lattice parameters a-axis and c-axis of hydroxyapatite unit cell having hexagonal lattice structure?

01 January 1970 9,978 2 View

Cyclooctatraene damaged by TFA?

16 February 2021 4,747 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Proteins not stacking/ entering in a native PAGE. Can anyone help?

Hi. I am trying to study a biotin modification of my target protein in a crude cell extract and am interested to determine how much of my protein in getting biotinylated. After biotinylating my...

15 January 2021 2,214 4 View

What buffer to use for biotinylation as alternative to PBS?

I'm looking to biotinylate a protein that precipitates in PBS. The recommended reconstitution buffer for the protein is urea/Tris buffer, pH 7,5 (7 M urea, 5 mM EDTA, 15 mM mercaptoethanol, 20 mM...

04 January 2021 7,681 5 View

What should be the ideal Thiolated-Oligo:GNP ratio for effective conjugation?

I need to conjugate thiolated oligos to GNPs and I am wondering what concentrations of the oligo to use with what concentration of GNPs for effective conjugation.

08 December 2020 7,975 2 View

What methods can I use to confirm if my oligo-GNP conjugate is hybridizing with my target nucleic acid?

Once I have oligo-GNP conjugates, I need to use them as sensor for my target nucleic acid. Before doing that I need to confirm whether the oligo probe (conjugated to GNP) hybridizes well with my...

04 December 2020 4,734 2 View

How to solve the problem with protein getting stuck in the wells during electrophoresis for EMSA?

26 November 2020 6,094 3 View

Have anyone tried to backbone digest Lenti-multi-guide with BsmBI for cloning?

Hi. I have planned to use the plasmid Lenti-multi-guide (Addgene Plasmid #85401). for mig cloning, and BsmBI as restriction enzyme...

21 October 2020 5,131 3 View

Can a 5'biotinylated PCR product be used in a PCR cloning kit ?

PCR cloning kits allow to clone a PCR product in a variety of vectors through a simple blunt-ended ligation. However, would this still work with PCR products containing modified ends (obtained...

21 October 2020 4,869 5 View

How to process fluorescently labeled oligos after HPLC purification?

Hello, I am purifyning fluorescent oligos through HPLC. My desired fraction of the mixture elutes at ~36% acetonitrile in triethylammonium acetate buffer, pH 7.0. I want these fractions to be...

23 September 2020 5,966 2 View