Determine the number of quails in a treatment/replication/pen?

How can we estimate the number of quail birds in a treatment and in a replication for 5 treatment and 4 replication for feed research? Is there any range limitation to keep the number of birds?

19 November 2020 2,856 3 View

List of Indian UGC listed journals?

Can anybody help me with the List of Indian UGC listed journals of Science Million thanks in anticipation

02 July 2020 9,671 2 View

Flood Inundation in HEC-RAS?

Hi, I am using HEC-RAS 5.0.7 to create Inundation Boundary, however, it seems HEC-RAS extends flood inundation boundaries up to Edge Lines (two sides of cross sections) and then shows the vertical...

27 October 2019 5,722 10 View

What is the best Machine Learning tool for time series analysis of physical-based simulations?

I am looking for a Machine Learning tool for Time series analysis. I have tried MATLAB (ANFIS tool) and ANN in R, but the error is more than my expectation in testing step. Furthermore, MATLAB...

07 December 2018 6,306 4 View

How to reform temperature distribution in simulation of a fillet weld in ansys?

19 August 2018 414 3 View

is it possible to use skin patch for insulin delivery in diabetic people?

is it possible to use skin patch for insulin delivery in diabetic people?

18 September 2016 7,404 3 View

Hello everyone! Can anyone provide me the Protocol for sample preparation to measure whole blood tacrolimus concentration using ELISA method?

16 February 2016 5,891 2 View

Adherent sf9 cell line or suspension sf9 ?

For sf9 vector transformation, almost all literature suggests to transform it when the cells is in adherent form. my cells are suspension in grace insect medium supplemented. Would you please...

02 February 2016 9,572 4 View

In transforming GUS control for DH10Bac Medium, which antibodies should we use?

Dear researchers In Bac to Bac kit for transforming control GUS plasmid in DH10Bac, which antibiotics should be used at agar plates? Is it the same as bacmid construct of our Gene of interest? It...

19 January 2016 5,255 3 View

How can I equilibrate a DLiPC membrane well at ambient temperature?

09 November 2015 1,068 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View