How can I separate large size plasmid (310kb) from gram positive bacteria bacillus anthracis?

More Fahimeh Mohammadi's questions See All

How much RNA should i prepare from my samples for sequencing?

Hi everyone I'm going to extract RNA for sequencing. I want to send the samples to the BGI company for sequencing. How much RNA should I prepare from my samples?

03 April 2018 4,792 3 View

Is there someone who guides me to extract a high quality RNA?

Hi everyone. I have to extract RNA from a tissue. Is there someone who guides me to extract a high quality RNA ? ( Important points that must be met, Suitable kit and protocol)

10 November 2017 8,940 8 View

Can someone introduce me an paper or website for whole genome sequencing?

Hi everyone I need an article or website for how whole genome sequencing of a species (Workflow, registration, annotation, materials and methods and etc). Can someone introduce me an article or...

09 October 2017 3,137 6 View

What method can be used to obtain the genome of a bacterium from water and milk that has been contaminated with the bacteria?

I'm going to use PCR method for amplification of a gene from a bacterium. my examples are water and milk That have been infected by a bacterium.Is there a special kit for the isolation of...

04 May 2017 9,445 8 View

How Be used from Circular Dichorism in the drug recombinant proteins?

Hello everyoneI need complete information about CD description in the drug recombinant proteinsIs there anyone who can help me?

31 December 2016 764 8 View

In your opinion, What is the best Human housekeeper genes for use in Liver tissue?

hi I am testing the housekeeper genes in the liver in humans, so I use a technique such as SYBR Green. In your opinion, what is the best genes?

31 December 2016 515 7 View

Is there general signal peptide for plants?

hi signal peptides are commonly protein-specific, I wonder if there is "general" signal peptides which can be used among plants. Hereby, it would enable more convenient protein expressions in...

31 December 2016 1,797 5 View

Is the Isoelectric Point of the capsid the same of the protein or does it change?

hi I am expressing a Virus Like Particle in Baculovirus System. The virus express a protein that self-assembles itself in a capsid. I know th IP of the protein. Since only one of three domains of...

31 December 2016 5,182 1 View

What is method of finding the cpg islands in promoter region of a gene?

hi I need to identify of cpg islands in the number of genes. Is there someone who guide me ؟

31 December 2016 314 4 View

Can anyone recommend a method to determine if bacteria are hup+ or hup-?

hiI want to measure N fixation as a H2 evolution with a specific device and I need to know if my strain has an active hydrogenase enzyme.

31 December 2016 4,246 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Dirk Schmidt

Hello Fahmeh,

you could try and seperate the two different kinds of DNA by electrophoresis and extract your plasmid DNA from the gel afterwards.

Best wishes

Dirk

Fahimeh Mohammadi

Thank you very much Mr. dirk

Md Hassanur Rahman

You can use gel extraction technique by any gel extraction kit protocol .

Good Luck