I have two bottles of cell culture media MEM Hanks and 199 Earls
I dont need them now, but i will later. How can I preserve them for about 6 months , without loss in nutrients or contamination ?
Hello Reham.
That is not a good idea to store media containing many different chemicals in them. However, if you must follow the procedure below.
Start from newly arrived fluidal or made from the powder formulation. Take great care of absolute sterile condition to make aliquots (50 or 100ml each in plastic container without serum without glutamate or pyruvate. Freeze them and store at -70C. When you need to use them, thaw in a water bath. Bring them to lamina flow. Supplement a half of Glutamate or puruvate if the media were used and stored at 4C for some time already. Otherwise, no need to supplement the energy sources. Millipore and add serum, followed by milipore again. They are ready for use.
Best wishes.
Hello Reham.
That is not a good idea to store media containing many different chemicals in them. However, if you must follow the procedure below.
Start from newly arrived fluidal or made from the powder formulation. Take great care of absolute sterile condition to make aliquots (50 or 100ml each in plastic container without serum without glutamate or pyruvate. Freeze them and store at -70C. When you need to use them, thaw in a water bath. Bring them to lamina flow. Supplement a half of Glutamate or puruvate if the media were used and stored at 4C for some time already. Otherwise, no need to supplement the energy sources. Millipore and add serum, followed by milipore again. They are ready for use.
Best wishes.
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