How can I measure exosome percent in my products? How can I understand these exosomes in my products are intact?
First, What the type of your products? and what the exosome percent you actually mean? Generally, exosomes should be purified and the particle concentration could be determined by some techniques e.g. NTA, and Nano-flow. In addition, the protein concentration of exosomes could be determined. Then, the percent is able to be calculated by division of the total number you want.
Dear Wang Jiang
Thank you so much for your kind answer. How do we measure the amount of exosome extracted and the amount of exosome inserted into the cream?
A gold standard for to asses whether exosomes are morphologically intact, you must perform cryogenic electron microscopy.
To measure exosome percentage (if you mean percentage in your crude exosomes preparation), you must purify it further using density gradient centrifugation to separate EVs from non-EV proteins and you can then calculate back the percentage of impurities vs exosomes.
the amount of exosomes could be determined by nanoparticle tracking analysis (NTA) or nano-flow-cytometry, and even the electron-microscopy. By these techniques, you may calculate the concentration of the exosome particles ( /mL). It is easy to refer to the methods of quantifying the virus particles or nano-particles. They used the similar tools for quantifiication.@Samane Attaran
- Badges
- Science topic