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Questions related from Daniele Campisi
Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...
17 October 2023 3,821 2 View
Hello! I tried to block my fixed cells on 10% FBS and 5% NGS (Normal Goat Serum) in PBS1X for 1h at RT and then incubate with a primary Ab anti-Mouse (4 °C ON in 10% of the blocking solution) and...
22 February 2022 5,617 6 View
I have marked my U87 glioma cells several times with a primary antibody made in rabbit and then revealed with Alexa 488 which marks a cytoplasmic protein and never had any problems with...
23 February 2021 6,106 4 View
Hello, I am interested in endocytosis from the plasma membrane and would like to block it. The problem is that, for example, a well-known molecule used for this purpose is "dynasor" which is a...
25 November 2020 5,375 3 View
I'm tracking endosomes inside the cell to graphically show an anterograte (towards the membrane) or retrograde (to the perinuclear zone) traffic. What I am doing is tracking edosomes along their...
28 June 2020 1,536 0 View
Hello! I bought this new reagent CellLight BacMan 2.0 for a protein fused to RFP to transduce my U87 glioblastoma cells and look at my moving proteins in live. I thought it was very efficient but...
30 January 2019 5,607 3 View
In order to extract protein from my cell lines I always used to destroy the cell lysate using syringe. After centrifugation I used to got a good pellet (cell debris and nuclear protein) in the...
21 May 2017 1,103 3 View
Hi! I have a problem with CXCR4 run. I should see the bands around 40 kDa but I see the staining at the beginning of the gel, I supposed that CXCR4 is not able to migrate. First time I tryed I...
20 June 2016 2,990 6 View
