Daniele Campisi

6 Questions 12 Answers 0 Followers

Questions related from Daniele Campisi

How to get rid of Streptavidin-HRP background/noise on westernblot?

Hello, we have been struggling with the lots of background in our wb membranes probed with an anti-Streptavidin-HRP from Thermofisher (Pierce 21134). Samples contained biotinilated proteins. Every...

17 October 2023 3,800 2 View

Blocking solution for IF with a Secondary Ab raised in Goat?

Hello! I tried to block my fixed cells on 10% FBS and 5% NGS (Normal Goat Serum) in PBS1X for 1h at RT and then incubate with a primary Ab anti-Mouse (4 °C ON in 10% of the blocking solution) and...

22 February 2022 5,607 6 View

How to get rid of non-specific nuclear signals in the staining of immunofluorescent microscopy?

I have marked my U87 glioma cells several times with a primary antibody made in rabbit and then revealed with Alexa 488 which marks a cytoplasmic protein and never had any problems with...

23 February 2021 6,093 4 View

How can I make a vector map with ImageJ of endosomal transport?

I'm tracking endosomes inside the cell to graphically show an anterograte (towards the membrane) or retrograde (to the perinuclear zone) traffic. What I am doing is tracking edosomes along their...

28 June 2020 1,522 0 View

Cell Light BacMan 2.0 in U87 glioma cells?

Hello! I bought this new reagent CellLight BacMan 2.0 for a protein fused to RFP to transduce my U87 glioblastoma cells and look at my moving proteins in live. I thought it was very efficient but...

30 January 2019 5,600 3 View

Problem with CXCR4 run in SDS-page, do proteins are not able to migrate?

Hi! I have a problem with CXCR4 run. I should see the bands around 40 kDa but I see the staining at the beginning of the gel, I supposed that CXCR4 is not able to migrate. First time I tryed I...

20 June 2016 2,982 6 View